Journal: PLoS ONE

Article Title: Development of a constant pressure perfused ex vivo model of the equine larynx

doi: 10.1371/journal.pone.0251530

Figure Lengend Snippet: A: The perfusion system (technical drawing) consists of a closed circuit. Red color indicates the hot water circuit, blue represents the perfusion circuit, gassed with carbogen. For active perfusion, the arteries were catheterized, passive venous outflow drains into the perfusion tub. The gassing tubes (one in the supply tank 1 and one in the perfusion tub) are denoted with an asterisk. The perfusion flow was measured using a flowmeter (F). In the inset photograph, a dorsal view of the perfusion setup in situ can be observed. The larynx, immersed in perfusion solution, is placed on a larynx retainer to stabilize its position in the perfusion tub. Both cranial thyroid arteries were catheterized (intravenous catheter, 18 gauge). For pH-control, a pH-meter is added (marked with an arrow). B: Schematic drawing of an equine larynx, lateral aspect (redrawn after our specimens and corrosions casts of the Institute of Veterinary Anatomy, Leipzig University). Relevant structures are the cricoarytenoideus dorsalis muscle [CAD, a)], the common carotid artery [b) running parallel to the external jugular vein], the catheterized arterial branch [c), cranial thyroid artery] followed by its ramification into the ascending pharyngeal artery (d) and the laryngeal branch (e). The latter two vessels nourish the CAD. f) Recurrent laryngeal nerve reaches the larynx as caudal laryngeal nerve.

Article Snippet: The perfusion solution was permanently gassed with Carbogen (95% O 2 , 5% CO 2 , Linde AG, Pullach, Germany).

Techniques: In Situ, Control